coli strain XL1-Blue and purified by affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl and 50 mM sodium phosphate, pH 8.0 (refs 11, 12, 18, 33, 53)
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These symptoms are usually temporary and resolve without medical intervention
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Ito, M., Shimizu, K., Niida, T., Amano, R